By Martin P Yurawecz
Content material: [v.1] The early years -- organic actions -- education of unlabeled and isotope-labeled conjugated linoleic and comparable fatty acid isomers -- advertisement construction -- Oxidation -- Methylation strategies -- Separation of conjugated fatty acid isomers -- fuel chromatography/(Electron influence) mass spectrometry research of conjugated linoleic acid (CLA) utilizing diverse derivatization innovations -- id of CLA isomers in meals and organic extracts through mass spectrometry -- affirmation of conjugated linoleic acid geometric isomers via capillary gasoline chromatography-Fourier remodel infrared spectroscopy -- Nuclear magnetic resonance spectroscopic research -- id and quantification of conjugated linoleic acid isomers in fatty acid combos by way of ¹³C NMR spectroscopy -- Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants -- Endogenous synthesis of rumenic acid -- impact of ionophores -- Species-dependent, sesonal, and nutritional version -- nutritional regulate of immune-induced cachexia -- Incorporation of conjugated fatty acid into organic matrices -- Bone metabolism -- danger of breast melanoma -- CLA in lipids of fish tissues -- CLA in human milk -- Lipid metabolism with regards to its anticarcinogenic task -- Conjugated linoleic acid metabolites in rats -- influence of conjugated linoleic acid on polyunsaturated fatty acid metabolism and immune functionality -- law of stearoyl-CoA desaturase by way of conjugated linoleic acid -- changing physique composition -- Feeding CLA to pigs: results on feed conversion, carcass composition, meat caliber, and palatability -- nutritional assets and intakes of conjugated linoleic acid consumption in people -- Formation, contents, and estimation of day-by-day consumption of conjugated linoleic acid isomers and trans-fatty acids in meals -- Experimental atherosclerosis in rabbits -- Modulation of diabetes via CLA -- Conjugated linoleic acid as a nutraceutical: 15 years of n-3 polyunsaturated fatty acid study -- melanoma inhibition in animals -- consumption of dairy items and breast melanoma possibility -- v. 2
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Additional info for Advances in Conjugated Linoleic Acid Research, Volume 2
Several useful reviews of CLA isomers are also listed, including general/ recent advances (8,16,17), a practical guide (18), methods of analysis after derivatization (19,20), and CLA in food (21)/milk fat (22)/milk/dairy products (23). Although most of these reviews are cursory at best, they do provide useful overviews of CLA composition and methods of analysis. The final part of this chapter will include such topics as application of semipreparative Ag-HPLC to isolate multigram quantities of CLA isomers or CLA-enriched TAG and problems in the development of standard methods utilizing Ag-HPLC.
2001) Analysis of CLA Derivatives by GC/MS, Eur. J. Lipid Sci. Technol. 103, 621–624. 31. G. (2002) Chromatographic Separation and Identification of Conjugated Linoleic Acid Isomers, Anal. Chim. Acta 465, 207–226. 32. Spitzer, V. ) pp. 110–125, AOCS Press, Champaign, IL. 33. -C. ) pp. 251–284, Marcel Dekker, New York. 34. W. (1989) Newly Recognized Anticarcinogenic Fatty Acids: Identification and Quantification in Natural and Processed Cheeses, J. Agric. Food Chem. 37, 75–81. 35. D. (1998) Factors Affecting the Conjugated Linoleic Acid Content of Cheddar Cheese, J.
Ultraviolet detection at 233 nm. (Published with permission from Ref. ) from Vol. I (14a). 1% ACN in hexane, Eulitz and co-workers (46) were able to separate as FAME the very difficult 9c,11t- and 9t,11c-18:2 isomer pair. Two to six columns connected in series were required to separate such isomer pairs as 8t,10c- from 7t,9c and 11c,13t- from 9t,13c-18:2. In comparison, analysis of this mixture by GC utilizing a 100-m, highly polar capillary column could separate the cis/cis, the cis/trans and trans/cis (incomplete separation) and trans/trans 11,1318:2 isomers, but could not resolve trans/trans 8,10-, 9,11- or 10,12-18:2 (47).